Find HS Code and trade information for abs isolation column

ABS Isolation Column

An ABS isolation column is a laboratory tool used in solid-phase extraction (SPE) and liquid chromatography for the separation and purification of biomolecules, particularly antibodies (Abs). It leverages the principle of affinity chromatography, employing a solid support matrix functionalized with a ligand that specifically binds to antibodies.

Material:

• Solid Support: Typically composed of porous beads made of polystyrene-divinylbenzene copolymer, often cross-linked for mechanical stability. Other materials like agarose or silica can also be used, though polystyrene-based resins are most common for antibody isolation due to their binding characteristics.
• Ligant: The key component is a protein, commonly Protein A, Protein G, or Protein L, covalently attached to the solid support. These proteins exhibit high affinity for the Fc region of various antibody isotypes. The choice of protein depends on the antibody source and desired selectivity.
  • Protein A: Binds strongly to the Fc region of most IgG antibodies from mammals (except mouse).
  • Protein G: Exhibits broader binding specificity than Protein A, binding to a wider range of IgG antibodies from various species.
  • Protein L: Shows high affinity for the light chains of antibodies, particularly those of the lambda isotype.
• Column Body: Usually polypropylene or similar inert plastic.

Purpose:

• Antibody Purification: Isolating antibodies from complex biological samples such as serum, plasma, cell culture supernatants, or ascites fluid.
• Antibody Concentration: Increasing the concentration of antibodies in dilute solutions.
• Antibody Detection: Preparing antibody samples for downstream analysis (e.g., ELISA, Western blotting).
• Immunoprecipitation: Isolating antibody-antigen complexes.

Function:

The column functions by selectively binding antibodies to the immobilized protein ligand. The sample is passed through the column, allowing antibodies to bind while unwanted components flow through. After washing away unbound impurities, the bound antibodies are eluted using a low pH buffer (e.g., glycine-HCl) or a high salt concentration, disrupting the antibody-ligand interaction.

Usage Scenarios:

• Research Laboratories: Commonly used in immunology, molecular biology, and biochemistry research.
• Biopharmaceutical Industry: Employed in the production and purification of monoclonal and polyclonal antibodies for therapeutic applications.
• Diagnostic Laboratories: Utilized for antibody enrichment prior to detection assays.
• Antibody Production Facilities: Integral to antibody manufacturing workflows.

Common Types:

• Protein A/G Columns: Combine Protein A and Protein G for broad antibody binding capacity.
• Protein A Columns: Preferred for IgG purification from mammalian sources (excluding mouse).
• Protein G Columns: Versatile option for a wider range of IgG antibodies.
• Protein L Columns: Ideal for isolating antibodies with lambda light chains.
• Pre-packed Columns: Convenient, ready-to-use columns with pre-loaded resin.
• Empty Columns: Allow users to pack their own resin for customized applications.
• Spin Columns: Small-format columns designed for rapid antibody purification using centrifugation.
• High Capacity Columns: Designed for processing large sample volumes.

Based on the provided information, “abs isolation column” can be classified under the following HS codes:

• 3926909910: This HS code covers “Other articles of plastics and articles of other materials of headings 3901 to 3914: Other: Other Laboratory ware”. Chapter 39 relates to plastics and articles thereof. Heading 3926 specifically covers articles of plastics and articles of other materials. The subheading 3926909910 further specifies “Other Laboratory ware”. Given that an “abs isolation column” is likely used in a laboratory setting, this HS code is applicable. The total tax rate is 42.8%, comprising a 5.3% base tariff and a 7.5% additional tariff, increasing to 30% after April 2, 2025.

• 3923900080: This HS code covers “Articles for the conveyance or packing of goods, of plastics; stoppers, lids, caps and other closures, of plastics: Other Other”. Chapter 39 pertains to plastics. Heading 3923 focuses on articles for conveyance or packing. The subheading 3923900080 denotes “Other Other”. If the “abs isolation column” is used for packing or conveying goods, this HS code could be relevant. The total tax rate is 58.0%, consisting of a 3.0% base tariff and a 25.0% additional tariff, rising to 30% after April 2, 2025.

• 8487900080: This HS code covers “Machinery parts, not containing electrical connectors, insulators, coils, contacts or other electrical features, and not specified or included elsewhere in this chapter: Other Other”. Chapter 84 relates to nuclear reactors, boilers, machinery and mechanical appliances. Heading 8487 covers parts of machinery. The subheading 8487900080 specifies “Other Other”. If the “abs isolation column” is a component of a larger machine, this HS code may be applicable. The total tax rate is 83.9%, including a 3.9% base tariff and a 25.0% additional tariff, increasing to 30% after April 2, 2025, with an additional 25% tariff applicable to steel or aluminum products.

• 8487900040: This HS code covers “Machinery parts, not containing electrical connectors, insulators, coils, contacts or other electrical features, and not specified or included elsewhere in this chapter: Other Oil seals, other than those of chapter 40”. Chapter 84 relates to nuclear reactors, boilers, machinery and mechanical appliances. Heading 8487 covers parts of machinery. The subheading 8487900040 specifies “Other Oil seals, other than those of chapter 40”. If the “abs isolation column” functions as an oil seal within a machine, this HS code could be considered. The total tax rate is 58.9%, consisting of a 3.9% base tariff and a 25.0% additional tariff, rising to 30% after April 2, 2025.

According to the provided reference material, the HS code options related to 'abs isolation column' are limited, with only the following 4 found.