Find HS Code and trade information for isolation column

Isolation Column

An isolation column is a laboratory tool used in analytical chemistry for the separation, purification, and concentration of target analytes from complex mixtures. It is a core component of solid-phase extraction (SPE), a sample preparation technique widely employed before instrumental analysis such as high-performance liquid chromatography (HPLC), gas chromatography (GC), and mass spectrometry (MS).

Material:

Isolation columns consist of a solid stationary phase packed within a column cartridge, typically made of polypropylene or polyethylene. Common stationary phases include:

• Silica-based phases: These are the most prevalent, offering a range of surface modifications to control polarity.
  • Reversed-Phase (RP): C18 (octadecyl), C8 (octyl), C4 (butyl) are common, utilizing hydrophobic interactions for separation.
  • Normal-Phase (NP): Silica, alumina, and diol phases employ polar interactions.
  • Ion-Exchange: Strong or weak cation or anion exchangers separate based on charge.
  • Mixed-Mode: Combine multiple interaction mechanisms (e.g., reversed-phase and ion exchange).
• Polymeric phases: Offer greater chemical stability and wider pH compatibility than silica-based phases. Polystyrene-divinylbenzene is a common example.
• Carbon-based phases: Graphitized carbon, used for separating polycyclic aromatic hydrocarbons and other non-polar compounds.

Purpose:

The primary purpose of an isolation column is to selectively retain target analytes while allowing unwanted matrix components to pass through or be washed away. This results in a cleaner, more concentrated sample suitable for downstream analysis, improving detection limits, accuracy, and reproducibility.

Function:

The separation process relies on the differential affinity of analytes for the stationary phase and the mobile phase (solvent). The process typically involves four key steps:

1. Conditioning: The stationary phase is prepared with a solvent to activate the binding sites.
2. Loading: The sample is applied to the column, allowing the target analytes to bind to the stationary phase.
3. Washing: Interfering compounds are removed by eluting them with a solvent that has a low affinity for the target analytes.
4. Elution: The target analytes are selectively removed from the column using a solvent with a high affinity for them.

Usage Scenarios:

Isolation columns are used in a wide variety of applications, including:

• Environmental monitoring: Extraction of pollutants from water, soil, and air samples.
• Pharmaceutical analysis: Purification of drugs and metabolites from biological fluids (e.g., plasma, urine).
• Food safety: Isolation of pesticides, herbicides, and mycotoxins from food matrices.
• Clinical chemistry: Sample preparation for biomarker analysis.
• Forensic science: Extraction of drugs of abuse and other evidence from biological samples.

Common Types:

Isolation columns are available in a variety of formats, differing in size, stationary phase, and binding capacity:

• Traditional SPE cartridges: The most common format, available in various sizes (e.g., 1 mL, 3 mL, 5 mL).
• Disk SPE: Larger surface area for higher capacity and faster flow rates.
• 96-well SPE plates: High-throughput format for automated sample preparation.
• Solid-Supported Extraction (SSE) columns: Use larger particle sizes and lower pressure, suitable for gravity-driven or vacuum-assisted extraction.
• QuEChERS columns: Specifically designed for quick, easy, cheap, effective, rugged, and safe (QuEChERS) sample preparation for pesticide analysis.

Based on the provided information, “isolation column” can be classified under the following HS codes:

• 3914002000: This HS code falls under Chapter 39: Plastics and articles thereof. Specifically, it covers Ion-exchangers based on polymers of headings 3901 to 3913, in primary forms: Cross-linked polyvinylbenzyltrimethylammonium chloride (Cholestyramine resin USP). This suggests the column utilizes a polymer-based ion-exchange resin for separation or purification.
• 3914006000: Also within Chapter 39: Plastics and articles thereof, this HS code covers Ion-exchangers based on polymers of headings 3901 to 3913, in primary forms: Other. If the isolation column utilizes a polymer-based ion-exchange resin but is not specifically cross-linked polyvinylbenzyltrimethylammonium chloride (Cholestyramine resin USP), this code applies.
• 3926909910: This HS code is categorized under Chapter 39: Plastics and articles thereof. It covers Other articles of plastics and articles of other materials of headings 3901 to 3914: Other: Other Laboratory ware. If the isolation column is made of plastic and is used as laboratory ware, this code is applicable.

Tariff Details (Based on Reference Material):

• 3914002000: Basic tariff: 0.0%, Additional tariff: 25.0%, Additional tariff after 2025.4.2: 30.0%. Total tariff: 55.0%.
• 3914006000: Basic tariff: 3.9%, Additional tariff: 25.0%, Additional tariff after 2025.4.2: 30.0%. Total tariff: 58.9%.
• 3926909910: Basic tariff: 5.3%, Additional tariff: 7.5%, Additional tariff after 2025.4.2: 30.0%. Total tariff: 42.8%.

Important Note:

Regarding HS code 3914002000 and 3914006000, the classification depends on the specific composition of the ion-exchange resin used in the column. If the column is specifically cross-linked polyvinylbenzyltrimethylammonium chloride (Cholestyramine resin USP), use 3914002000. Otherwise, use 3914006000.